The 2022 European Congress on Alternatives to Animal Testing

Replacement

• Disease models and microphysiological systems
• Emerging technologies: stem cells incl. hiPSCs, bioprinting, spheroids, organoids, multi-organ models, “-omics” analysis, systems biology approaches, IT and big data
• Biological barriers: e.g. lung, gut, kidney & skin epithelia, blood-brain & blood-saliva barriers
• Animal-free human based cell culture – replacement of serum, matrigel, gelatin, collagens, trypsin, S9, etc. – and recombinant antibodies
• Good cell culture practice
• Development and implementation of Novel Approach Methodologies (NAMs)
• In silico models: toxicology & efficacy of drugs, chemicals & cosmetics, new approaches for biomedical research
• In vitro toxicology: Specific endpoints of toxicity, alternatives to animal testing in food safety and nutrition, Neurotoxicity & Developmental Neurotoxicity (DNT) and Ecotoxicology
• Efficacy and safety testing of drugs, medical devices, biopharmaceutics, cosmetics, consumer products (e. g. toys) and chemicals (REACH & alternatives)

Reduction

• Preregistration, reporting, and experimental design
• Organ and tissue sharing and approaches to reduce surplus animals
• Translational aspects (species differences) and reproducibility

Refinement

• Best practice approaches and culture of care
• Avoidance of severe suffering and pain management/assessment
• Cage enrichment and non-invasive handling

• COVID-19 pandemic and the 3Rs: in vitro models, vaccine development and drug testing
• 3Rs in education and academia
• 3Rs centres and platforms in Europe & international, national and local centres
• 3Rs and policy: Implementing 3Rs guidelines and concepts, Novel Approach Methodologies (NAMs) and Directives – Ethical and legal issues – Cosmetics testing ban (and REACH)
• “Young Scientists” session
• Publication policies – ARRIVE guidelines & Co

Monday, 26th September 2022

10:00: Start of Registration

11:30 – 12:30: YOU Event – Meet-up for Young Scientists (Hall 3)

13:00 – 13:15: Opening

13:15 – 13:45: Keynote lecture: Susanna Louhimies (European Commission) “Directive 2010/63/EU – Accelerating the transition towards less animals and better science” (Hall 1), Chairs: Winfried Neuhaus & Horst Spielmann

14:00 – 15:30: Scientific sessions in Hall 1, Hall 2, Hall 3

Hall 1 – HS 1 (ground floor): Covid-19 pandemic and the 3Rs

Chairs: Merel Ritskes-Hoitinga & Dilyana Filipova

14:00 – 14:20: Merel Ritskes-Hoitinga: “Never waste a good crisis: Case study revealed faster regulatory approval of COVID-19 vaccine with fewer animal studies and more alternatives. Let’s continue this promising road.”

14:20 – 14:40: Stefan Hippenstiel: “Human lungs show limited permissiveness for SARS-CoV-2 due to scarce ACE2 levels but virus-induced expansion of inflammatory macrophages”

14:40 – 15:00: Stefanie Dichtl: “Cilgavimab/Tixagevimab as alternative therapeutic approach for BA.2 infections”

15:00 – 15:15: Viktoria Zaderer: “Optimizing animal-free 3D lung model for SARS-CoV-2 research and drug testing”

15:15 – 15:30: Dilyana Filipova: “Role of non-animal technologies in COVID-19 research”

Hall 2 – HS 2 (ground floor): NAMs for Developmental Neurotoxicity

sponsored by

Chairs: Ellen Fritsche & Martin Paparella

14:00 – 14:20: Martin Paparella: “An analysis of the limitations and uncertainties of in vivo developmental neurotoxicity testing and assessment to identify the potential for non-animal-methods approaches”

14:20 – 14:40: Ellen Fritsche: “Human Test Methods for Developmental Neurotoxicity (DNT) Evaluation: Set-up, Scientific Validation and Statistical Analyses”

14:40 – 15:00: Iris Mangas: “Lessons Learned from EFSA AOP informed IATA Case Studies Integrating in vitro data for DNT Risk Assessment”

15:00 – 15:15: Katharina Koch: “An in-vitro approach to assess endocrine modes of action leading to developmental neurotoxicity”

15:15 – 15:30: David Pamies: “Human IPSC-Derived Model to Study Myelin Disruption”

Hall 3 – Seminar room (first floor): Preregistration/Best Practise

Chairs: Arti Ahluwalia & Györgyi Szabó

14:00 – 14:20: Jan Lauwereyns: “Tracking the Validity of Animal Models for Biomedical Research”

14:20 – 14:40: Lisa Grohmann: “Sharepoint Refinement: Internal platform for improved Refinement implementation”

14:40 – 15:00: Julia M. L. Menon: “Preregistration of animal research protocols: insights into preclinicals.eu 3-years development” – YSTA

15:00 – 15:15: Céline Heinl: “Preregistration in animal research – Animal welfare and scientific progress”

15:15 – 15:30: Sabine J. Bischoff: “Increase the quality of animal research with CIRS-LAS – Research before the study, report during the study and discuss after the study”

15:30 – 16:00: Coffee break

16:00 – 17:30: Scientific sessions in Hall 1, Hall 2, Hall 3

Hall 1 – HS 1 (ground floor): 3Rs Policy

Chairs: Susanna Louhimies & Horst Spielmann

16:00 – 16:20: Sandra Coecke: “How Food Science, Next Generation Life Science Methods and Artificial Intelligence relates to European regulatory decision-making on food systems for sustainable health?”

16:20 – 16:40: Laurence Walder: “Improving Member States reporting on the implementation of Directive 2010/63/EU”

16:40 – 17:00: Samantha Saunders: “The RAT list: a tool for highlighting areas of animal use ready for replacement”

17:00 – 17:15: Mathieu Vinken: “The European ONTOX project: safer chemicals using less animals”

17:15 – 17:30: Susanna Louhimies: “Education and training – an integral part of the Three Rs’ toolbox”

Hall 2 – HS 2 (ground floor): Novel Technologies

Chairs: Nathalie Jung & Jordi Grifols Ronda

16:00 – 16:20: Philipp Paulitschke: “New non-invasive, label-free monitoring approach for high cell culture and assay quality”

16:20 – 16:40: Nathalie Jung: “Enabling non-invasive and chemically-selective analysis of organoid cultures using confocal Raman microscopy”

16:40 – 17:00: Nicole Guazzelli: “Integrated Strategies for the Design of Core-Shell Structures as Advanced Barrier Models” – YSTA

17:00 – 17:15: Sebastian Eggert: “cellasys #8: A microphysiometric test to identify serum-free cell culture media”

17:15 – 17:30: Katrin Colleselli: “An optogenetic approach to characterise human TLR2 homo, -and heterodimers in THP-1 and THP-1 derived macrophages” – YSTA

Hall 3 – Seminar room (first floor): Refinement

Chairs: Adrian Smith & Annemarie Lang

16:00 – 16:20: Adrian Smith: “An overview of guidelines for better preclinical research”

16:20 – 16:40: Giuseppe Chirico: “An in-vivo engineered chip as a smart intravital multiphoton imaging window for new validation protocols of biomaterials”

16:40 – 17:00: Kerstin E. Auer: “Effect of group size and cage cleaning interval on male aggression level and wellbeing in two mouse strains

17:00 – 17:15: Angelique Wolter: “Evaluation of a new microparticulate depot formulation of buprenorphine for sustained post-surgical analgesia” – YSTA

17:15 – 17:30: Jürgen Alphonsus: “Development of 3D-printed surgical guides to standardize, refine and reduce animal experiments in osseointegration research (The ‘PRECISE’ project)”

18:00 – 19:00: Round table/panel discussion: “The rise and role of 3Rs centres in Europe” (Hall 1 – HS1 ground floor)

19:00 – 21:00: Welcome Reception (Aula)

20:00 – 21:00: YOU Event – Meet the Mentor (Hall 3 – Seminar room first floor)

Tuesday, 27th September 2022

9:00 – 9:30: Keynote lecture: Merel Ritskes-Hoitinga (University of Utrecht, NL) “Evidence-based transition to animal-free innovations: let’s make it happen!” (Hall 1), Chairs: Kristina Wagner & Doris Wilflingseder

9:40 – 11:10: Scientific sessions in Hall 1, Hall 2, Hall 3

Hall 1 – HS 1 (ground floor): 3Rs Policy: Accelerating the Transition to Innovation without the Use of Animals

Chairs: Kristina Wagner & Doris Wilflingseder

09:40 – 10:00: Luísa F. Bastos: “Transition to non-animal science – Current state of play in the EU”

10:00 – 10:20: Ingrid J. Visseren-Hamakers: “Accelerating the transition to animal-free innovation: A transformative governance approach”

10:20 – 10:40: Iris Mangas: “Implementing New Approach Methodologies in EFSA’s Next Generation Risk Assessment”

10:40 – 10:55: Evangelos Daskalopoulos: “Innovative models in biomedical research”

10:55 – 11:10: Lisa Hara Levin: “Outcomes from a Multistakeholder Engagement to Address the Challenges Associated with the Development, Regulatory Approval, and Implementation of Non-Animal Methods in Safety Testing and Biomedical Research”

Hall 2 – HS 2 (ground floor): Replacement: Lung in vitro Models

Chairs: Claus-Michael Lehr & Stefan Hippenstiel

09:40 – 10:00: Pauline Zamprogno: “IPF-on-chip: An alternative to animal models” – YSTA

10:00 – 10:20: Léa Todeschini: “A barrier-on-chip model with integrated 3D stretching to support preclinical decision making”

10:20 – 10:40: Michelle Mueller: “Human induced pluripotent stem cell-derived alveolar epithelial cells: a comparative gene and protein expression analysis between 2D- and 3D-differentiation”

10:40 – 10:55: Lorenz Latta: “AELVi “Arlo” – A novel monoclonal human alveolar epithelial cell line with reproducible barrier function”

10:55 – 11:10: Vanessa Almonti: “Evaluation of cytotoxicity and pro-inflammatory effects of Russian chrysotile fibres on the EpiAirway In Vitro Human Airway Model”

Hall 3 – Seminar room (first floor): NAMs for Risk Assessment

Chairs: Helena Kandarova & Kristin Schirmer

09:40 – 10:00: Jan Markus: “Development of physiologically relevant in vitro inhalation model to predict acute respiratory toxicity of mists and volatile liquids”

10:00 – 10:20: CANCELLED Caroline Frädrich: “Identifying endocrine disruptors via a HTS platform based on the Sandell-Kolthoff reaction”

10:20 – 10:40: Kristin Schirmer: “Fish cell lines as animal-free and resource-efficient alternatives to fish in environmental risk assessment”

10:40 – 10:55: Ignacio J. Tripodi: “Turning AOPS into testable hypotheses with natural language processing and tissue-specific knowledge graphs”

10:55 – 11:10: Mieke Van Mulders: “RE-Place: stimulating knowledge sharing on New Approach Methodologies via an open access database in Belgium”

11:10 – 11:40: Coffee break

11:40 – 13:10: Scientific sessions in Hall 1, Hall 2, Hall 3

Hall 1 – HS 1 (ground floor): 3Rs Centers – International

Chairs: Winfried Neuhaus & Yasuyuki Sakai

11:40 – 12:00: Winfried Neuhaus: “Updates of the EU3Rnet”

12:00 – 12:20: Yasuyuki Sakai: “Latest activities of JSAAE and Asian organization”

12:20 – 12:40: Stefan Hippenstiel: “Einstein Center 3R – a joint initiative of Berlin research institutions”

12:40 – 12:55: Arti Ahluwalia: “The challenges and successes of promoting 3Rs in teaching and research in Italy: a Centro 3R update”

12:55 – 13:10: Birgit Reininger-Gutmann: “A3RC – Austrian 3R Center Update”

Hall 2 – HS 2 (ground floor): Cancer Models

Chairs: Giuseppe Chirico & Anna Sebestyén

11:40 – 12:00: Michael J. Ausserlechner: “3D bioprinted, vascularized neuroblastoma microenvironment for studying tumor-angiogenesis and metastatic processes in fluidic chip devices”

12:00 – 12:20: Tine Haesen: “Ex Vivo Human Precision-Cut (Tumor) Lung Slices as a Tool for the Evaluation of CD109 in Non-Small-Cell Lung Cancer”

12:20 – 12:40: Yuval Daskal: “An Automated Platform for Creating Patient-Derived Glioblastoma Organoids and High Throughput Drug Screening” – YSTA

12:40 – 12:55: Djenana Vejzovic: “Short and long term patient-derived sarcoma models – to study the variety of soft-tissue tumors”

12:55 – 13:10: Beate Rinner: “Protein Arginine Methylation as a therapeutic Target on patient derived autologous Clear Cell Sarcoma Model”

Hall 3 – Seminar room (first floor): Ecotoxicolgy

Chairs: Christopher Faßbender & Stefan Scholz

11:40 – 12:00: Stefan Scholz: “A Bayesian Network tool for Predicting Fish Acute Toxicity – can simple behaviour tests in fish embryos used to increase confidence in predicted effect concentrations?”

12:00 – 12:20: Cinta Porte: “Development of fish liver PLHC-1 spheroids and its applicability to investigate the toxicity of plastic additives”

12:20 – 12:40: Julia Nöth: “Screening of vascular disruptors as an endpoint to predict developmental toxicity using zebrafish embryos”

12:40 – 12:55: Inska Reichstein: “Application of a Biotechnological Metabolisation System to Improve the Forecast Power of the Fish Embryo Acute Toxicity Test (FET)”

12:55 – 13:10: Kerstin Schirmer: “Using machine learning to predict chemical toxicity”

13:10 – 14:00: Lunchtime including Business lunch sponsored by RepRefRed

14:00 – 15:30: Poster session 1 (first floor)

15:30 – 17:00: Scientific sessions in Hall 1, Hall 2, Hall 3

Hall 1 – HS 1 (ground floor): Stem Cell Models

Chairs: Jürgen Hescheler & Bettina Seeger

15:30 – 15:50: Elodie Vandenhaute: “Bridging the gap between in vitro & in vivo testing: 3D cell culture taking into account the extracellular matrix”

15:50 – 16:10: Kristina Bartmann: “Assessment of human neuronal network formation and function using 2D and 3D hiPSC-derived cell systems”

16:10 – 16:30: Lisa Nguyen: “Puromycin aminonucleoside induces kidney injury in iPSC-derived kidney organoids”

16:30 – 16:45: Jördis Klose: “Phenomics and transcriptomics applied for key event identification resulting in an AOP network for developmental neurotoxicity”

16:45 – 17:00: Bettina Seeger: “High sensitivity of stem cell-derived motor neurons to pharmaceutically relevant Botulinum Neurotoxins A1, B1, E1 and F1”

17:00 – 17:15: Julia Kapr: “In vitro disease modeling of the Cockayne Syndrome B neuropathology using patient-derived iPSC – a 3R implementation approach” – YSTA

Hall 2 – HS 2 (ground floor): In silico Toxicology

sponsored by

Chairs: Arti Ahluwalia & Ashwani Sharma

15:30 – 15:50: Arti Ahluwalia: “In silico methods: empowering and mainstreaming alternative methods”

15:50 – 16:10: Ashwani Sharma: “Machine learning based drug-induced Genotoxicity prediction model by evaluating physiochemical descriptors: Artificial Intelligence (AI) Approach”

16:10 – 16:30: Pedro Matos-Filipe: “Building virtual cohorts via the integration of public data”

16:30 – 16:45: Elena Lo Piparo: “Increasing confidence in NAMs through the integration of in silico and in vitro data: Pyrrolizidine Alkaloid (PA) case study”

16:45 – 17:00: Ermes Botte: “Advanced in silico approaches for designing human-relevant organ models” – YSTA

17:00 – 17:15: Vijay K. Gombar: “OrbiTox – Interactive Visualization of Multi-domain Experimental and Predicted Data for Translational Discovery”

Hall 3 – Seminar room (first floor): Liver & Intestine Models

Chairs: Gabriele Scholz & Armin Wolf

15:30 – 15:50: Armin Wolf: “The human 3D liver spheroid model is a pragmatic DILI hazard identification approach for early drug discovery phases”

15:50 – 16:10: Tim Kaden: “Evaluation of drug-induced liver toxicity of trovafloxacin and levofloxacin in a human microphysiological liver model” – YSTA

16:10 – 16:30: Valerie Beneke: “Microbiota dependent modulation of immune mediators in intestinal tissue slices ex vivo”

16:30 – 16:45: Brigitta Loretz: “Leaky Gut Model for the Investigation of Productive Delivery of JAK-1 siRNA”

16:45 – 17:00: Nathalie Jung: “Tuning tissue characteristics and complexity in in vitro models of the human intestinal mucosa”

17:00 – 17:15: Sabrina Schnur: “In vitro assay-based drug efficacy testing for the treatment of Inflammatory Bowel Disease (IBD)”

17:15 – 17:45: Coffee break

17:45 – 19:15: Prof. Horst Spielmann Session, Hall 1 – HS 1 (ground floor)

Chair: Winfried Neuhaus

• Introduction
• Horst Spielmann: “Pluripotent stem cell assays: modalities and applications for predictive embryotoxicity”
• Jürgen Hescheler: “The application of the stem cell concept in basic and applied research in the life sciences”
• Ellen Fritsche: “Horst Spielmann’s foster child: the human DNT in vitro tests”
• Helena Kandarova: “Difficult roads often lead to beautiful destinations – lessons learnt at ZEBET”
• Malte Spielmann: “The contribution of single cell technologies to the 3Rs”

20:00 – 24.00: Gala dinner in Neues Rathaus

Wednesday, 28th September 2022

9:00 – 9:30: Keynote lecture: Sasha Mendjan (IMBA, AUT) “Cardioids unravel human heart development and defects” (Hall 1), Chairs: Martin Paparella & Markéta Dvořáková

9:40 – 11:10: Scientific sessions in Hall 1, Hall 2, Hall 3

Hall 1 – HS 1 (ground floor): Non-animal Methods for Cardiac Toxicity

Chairs: Martin Paparella & Ronette Gehring

09:40 – 10:00: Martin Paparella: “Towards an Integrated Approach to Testing and Assessment for cardiotoxicity”

10:00 – 10:20: Monica Boffito: “Designing 3D bioengineered in vitro cardiac tissue models as reliable tools for the evaluation of chemical cardiotoxicity”

10:20 – 10:40: Edoardo Luca Viganò: “Computational modelling for cardiac toxicity”

10:40 – 10:55: Ronette Gehring: “The role of physiologically-based kinetic models within the new approach methodologies framework for predicting cardiotoxicity”

10:55 – 11:10: Mohammad Ghosheh: “The Electro-Mitochondrial Coupling of a Microphysiological Human Heart” – YSTA

Hall 2 – HS 2 (ground floor): Biological Barrier Models for Infection Studies

Chairs: Claus-Michael Lehr & Jens Kurreck

09:40 – 10:00: Claus-Michael Lehr: “Modelling Biological Barriers for Improving the Delivery of Novel Anti-infectives”

10:00 – 10:20: Jana Wächter: “A novel three-dimensional in vitro biofilm model in combination with human tissues – towards predictive chronic infection modeling” – YSTA

10:20 – 10:40: Jens Kurreck: “Bioprinting of Organ Models for Infection Studies”

10:40 – 10:55: Samy Aliyazdi: “3D-(Bio-) Printing for Advanced in Vitro Systems to Investigate Nano-Antibiotics against Bacterial Infections”

10:55 – 11:10: Mareike Ahrends: “Effect of CDE in RSV-infected human lung tissue ex vivo as alternative for animal-free testing of antiviral therapeutics”

Hall 3 – Seminar room (first floor): 3Rs and Education

Chairs: Christa Thoene-Reineke & David Pamies

09:40 – 10:00: Nick D. Jukes: “‘DVM: Training the Animal Doctor’ – a documentary film series”

10:00 – 10:20: Julia Menon: “Young TPI: the Dutch initiative for empowering the new scientific generation to go animal-testing-free” – YSTA

10:20 – 10:40: Lea Benner: ” “Improve your Skills” – the innovative 3R-Skills Lab of the 3R-Centre Giessen”

10:40 – 10:55: David Pamies: “Promoting 3Rs Public Awareness in a Young Audience”

10:55 – 11:10: Maximilian Nuber: “Modeling the In Utero Electroporation and surgical techniques beyond for the Replacement of animals in teaching”

11:10 – 11:40: Coffee break

11:40 – 13:10: Scientific sessions in Hall 1, Hall 2, Hall 3

Hall 1 – HS 1 (ground floor): Replacement – 3D in vitro Models

sponsored by

Chairs: Winfried Neuhaus & Horst Spielmann

11:40 – 12:00: Glauco R. Souza: “Magnetic 3D Bioprinting as a Surrogate to Aorta Ring Assay”

12:00 – 12:20: Dongwei Wu: “3D bioprinted cancer model as an efficiently applicable drug testing platform” – YSTA

12:20 – 12:40: Antonella Cecchettini: “A dynamic in-vitro model of smooth muscle and endothelial cells cultures for vascular calcification study”

12:40 – 12:55: Moritz Pfeiffenberger: “Development of an innovative cartridge bioreactor for parallelized cultivation and stimulation of complex tissue models”

12:55 – 13:10: Tobias Weigel: “3D multi-layered epithelial models based on animal free fibrous scaffolds”

Hall 2 – HS 2 (ground floor): Replacement of Animal-derived Products

Chairs: Christopher Faßbender & Jan van der Valk

11:40 – 12:00: Tilo Weber: “Animals in the (Petri) Dish: Towards a Truly Animal-free Laboratory”

12:00 – 12:20: Manuela Cassotta: “A worldwide survey on the use of animal-derived materials and reagents in scientific experimentation”

12:20 – 12:40: Andreas Schiwy: “Animal-free in vitro – Replacement of animal-derived products within the OECD TG 487 and 455 – Phase 1 report”

12:40 – 12:55: Johannes Hackethal: “Perspectives and Challenges by use of Human Placenta derived Biomaterials in Cell Culture and Regenerative Medicine”

12:55 – 13:10: Barbara Jozef: “A serum-free medium that supports cultivation of fish cell lines: case study about RTgill-W1 going serum-free” – EPAA Student grantee

Hall 3 – Seminar room (first floor): 3Rs Centres and Implementation

Chairs: Kristina Kejlová & Arti Ahluwalia

11:40 – 12:00: Markéta Dvořáková: “3Rs Centre Czech Republic at National Institute of Public Health in Prague”

12:00 – 12:20: Karin Schmelz: “Evaluation of Charité 3R measures to implement 3Rs in biomedical research at Charité using online surveys and personal interviews”

12:20 – 12:40: Melissa K. Valussi: “3R-SMART: Schooling Platform for Methodological Approaches to Reduce Animal Tests”

12:40 – 12:55: Mieke Van Mulders: “The Innovation Center for 3Rs (IC-3Rs) in Belgium”

12:55 – 13:10: Sara Capdevila: “Implementation of the 3Rs program in the Comparative Medicine and Bioimage Centre of Catalonia.”

13:10 – 14:00: Lunchtime including Business lunch sponsored by BioTechne

14:00 – 15:30: Poster session 2 (first floor)

15:30 – 17:00: Scientific sessions in Hall 1, Hall 2, Hall 3

Hall 1 – HS 1 (ground floor): Skin and Eye in vitro Models

Chairs: Silvia Letasiova & Kristina Wagner

15:30 – 15:50: Franciska Erdő: “Comparative analysis of chemical composition and permeability properties of artificial and excised skins – Reduction and Replacement”

15:50 – 16:10: Marek Puskar: “An epidermal model containing melanocytes for skin pigmentation and lightening studies.”

16:10 – 16:30: Tommaso Sbrana: “A new highly representative in-vitro model of human skin”

16:30 – 16:45: Silvia Letasiova: “Identification and Sub-categorization of Ocular Irritants Using the EpiOcular Tissue Model – Prediction Models for Liquids and Solids”

16:45 – 17:00: Nicola Knetzger: “Impedance Spectroscopy as tool to determine concentration-dependent eye irritation effects”

Hall 2 – HS 2 (ground floor): 3Rs Projects: Quality Aspects and Funding

Chairs: Anna Maria Bassi & Rebecca Ram

15:30 – 15:50: Maren Hülsemann: “Quality enhancing measures in organ model research”

15:50 – 16:10: Jens Kurreck: “Animal Components in Life Sciences and their Alternatives”

16:10 – 16:30: Melanie-Jasmin Ort: “The relevance of human primary material in animal free research – orthopedic trauma as an example”

16:30 – 16:45: Rebecca Ram: “Lush Prize 2012- 2022 and beyond: Supporting human relevant, animal-free research”

16:45 – 17:00: Herwig Grimm: “Advancing 3Rs: The National Research Programme 79 of the Swiss National Science Foundation”

Hall 3 – Seminar room (first floor): Human Disease Models

Chairs: Paula Braun & Christian Lotz

15:30 – 15:50: Christian Lotz: “Focus on efficacy: In vitro tissue models for preclinical research”

15:50 – 16:10: Eva I. Reihs: “Bioengineering the inflammatory and degradative cross talk environment in arthritic diseases with patient-derived heterotypic joint-on-a-chip systems” – YSTA

16:10 – 16:30: Michael J Burgum: “Developing new-approach in vitro toxicology methods to elucidate the DNA damage mechanisms of nanofibres”

16:30 – 16:45: Lilas Courtot: “The Future of Parkinson’s Disease Research: A New Paradigm of Human-Specific Investigation Is Necessary”

16:45 – 17:00: Dilyana Filipova: “No improvement in 60 years: drug failure rates from the 1960s to the 2010s”

17:15 – 18:30: YSTA Awards/Announcement WC12/Farewell

Poster Session 1

Tuesday, 27.9.2022, 14:00 – 15:30, first floor

Poster 1
Investigation of ADHD-associated alterations of the neurovascular unit by aid of hiPSC-derived in vitro models
Markus Glaser, Zora Schickardt, Sabrina Oerter, Rhiannon McNeill, Carolin Koreny, Christian Lotz, Sarah Kittel-Schneider, Antje Appelt-Menzel

Poster 2
Identifying skin sensitizers with an innervated 3D-skin model
Wiebke Bergforth (YSTA), Maren Schenke, Bettina Seeger

Poster 3
Rational design and evaluation of electrospun scaffolds as cell culture substrates for the cultivation of human induced pluripotent stem cells
Felix Rohde (YSTA), Karin Danz, Nathalie Jung, Sylvia Wagner, Maike Windbergs

Poster 4
Penetration of topical aminophosphonates through the dermal barrier – “skin-on-a-chip” development with an emphasis on animal number reduction
Dorottya Kocsis (YSTA), Rusul Keshwan, Franciska Erdő

Poster 5
Dry powder aerosols crossing the pulmonary barrier in vitro – Powder Atomizer aims for predicting human pharmacokinetic profiles
Marius Hittinger, Janina Osti, Annika Dehne, Sarah Wengschick, Thorsten Preisegger, Daniel Primavessy, Marielle Fink, Marc Mamier, Tobias Krebs

Poster 6
Characterization of human brain endothelial cells for the development of in vitro blood-brain barrier models.
Natascha Santacroce, Floriana Burgio, Laura Suter-Dick

Poster 7
Immunocompetent 3D model of human airways as predictive tool for clinical outcomes
Martina Di Fede, Giulia Realini, Bruna Clemente, Serena Tondi, Marianna Taccone, Sonia Budroni, Martina Canè, Andrea Ariolli, Kevin Pete Buno, Francesca Schiavetti, Michela Brazzoli, Daniela Rinaudo, Isabel Delany, Raffaella Cecchi, Tommaso Pasquali, Shahjahan Shaid, Silvia Rossi Paccani, Alfredo Pezzicoli

Poster 8
Using an innovative in vitro platform to investigate the early effects of oxidative stress and increased pressure on human trabecular meshwork cells.
Sara Tirendi, Anna Maria Bassi, Vanessa Almonti, Sonia Scarfì, Mario Passalacqua, Stefania Vernazza

Poster 9
Interaction and transport of small extracellular vesicles across human biological barrier in vitro models
Adrián Klepe, Ana Špilak, Sophia Theresa Kriwanek (YSTA), Andreas Brachner, Christa Nöhammer, Winfried Neuhaus

Poster 10
Animal-Free Plant-Derived Nanofibrillar Cellulose Hydrogels for Cell-Based Assays with Stem Cell and Organoid Cultures
Lauri Paasonen, Essi M. Niemi, Jonathan Sheard, Tony Kiuru

Poster 11
Humanized 3D cell culture materials – the next generation of in vitro testing
Catarina Custódio, Inês Deus, Cátia Monteiro, Sara Santos, João Mano

Poster 12
Human platelet lysate as a replacement for fetal bovine serum in biological barrier in vitro models
Andreas Brachner, Christina Gruber, Malgorzata Burek, Anja Neuhoff, Claudia Bernecker, Peter Schlenke, Winfried Neuhaus

Poster 13
Using perinatal tissues as a sustainable source of xeno-free platforms for cell culture
Inês Araújo Deus (YSTA), Catarina Almeida Custódio, João Filipe Mano

Poster 14
Fostering Awareness, Accessibility, and Acceptance of Animal-Free Antibodies
Niamh Haslett, Michael Cook, Jesus Calvo-Castro, Stewart Kirton, Jarrod Bailey

Poster 15
Lysosomal trapping measurement in multiple cell types using High Content Screening System
Johanna Pacsuta, Katalin Jemnitz, Gábor Nagy, Zsuzsanna Gáborik

Poster 16
Risk assessment of medical devices for the oral cavity using a human oral mucosa epithelium model
Ana Špilak, Peter Pôbiš, Lada Svobodová, Alena Moulisová, Andreas Brachner, Markéta Dvořáková, Helena Kandarova, Dagmar Jirová, Kristina Kejlová, Winfried Neuhaus

Poster 17
Landscape New Approach Methodologies (NAMs) for safety assessment of chemical substances
Victoria C. de Leeuw, Jelle Vriend, Lianne de Wit-Bos, Jacqueline van Engelen, Anne Kienhuis

Poster 18
Effects of nanoplastics on cells of the neurovascular unit
Aleksandra Chmielewska, Marie-Therese Leiningen-Westerburg, Lukas Wimmer, Lea Ann Dailey, Malavika Manju Sudheer, Despina Fragouli, Ana Špilak, Andreas Brachner, Winfried Neuhaus

Poster 19
Generation of reporter models for rapid oxidative stress induction measurement in vitro and application to 3D bronchial tissue cultures
Marion Alriquet, Giulia Fonti, Karsta Luettich, Marco van der Toorn, Stefan Frentzel, Joanne Chua

Poster 20
Use of organotypic gingival cultures for the assessment of nicotine pouches
Filippo Zanetti, Marion Alriquet, Laura Ortega Torres, Laurent Neau, Fabio Maranzano, Claudius Pak, Amandine Schmutz, Carole Mathis, Nazan Gunduz, Joanne Chua

Poster 21
Reducing the number of controls in fish early life stage toxicity tests when solvents are used
Christopher Faßbender, John W Green, John Handley, Thomas H Hutchinson, Lennart Weltje, Daniel Faber, Gilly Stoddart

Poster 22
Evaluation of the cytotoxic and inflammatory effect of two size-separated fractions of Russian chrysotile asbestos fibre
Serena Mirata, Vanessa Almonti, Stefania Vernazza, Dario Di Giuseppe, Alessandro F. Gualtieri, Anna Maria Bassi, Sonia Scarfì

Poster 23
Evaluating the toxicity of sea-dumped conventional and chemical munitions to fish and human cells using a combination of cell viability assays
João Barbosa, Colin R Janssen, Jana Asselman

Poster 24
Optimization of skin sensitization testing strategy in vitro for medical device extracts
Lada Svobodová, Marian Rucki, Alena Vlkova, Kristina Kejlova, Dagmar Jirova, Hana Kolarova

Poster 25
Prediction of human toxicology with alternative experimental models – zebrafish embryo (EU project PrecisionTox)
Stefan Scholz, Riccardo Massei

Poster 26
Risk assessment of microplastic by in vitro genotoxicity assays
Clemens Rausch, Elisabeth Simböck

Poster 27
Hormonal influence on the primordial germ cell proliferation and distribution in the early development of the Medaka
Leonie Hobohm, Lukas Strobel, Annika Pätzold, Alexander Froschauer

Poster 28
Effects of Plastic on the reproductive behaviour of the freshwater snail Biomphalaria Glabrata: an invertebrate in vivo study
Linda Prähauser, Robin Friedrich, Nevena Lakic, Karoline Schweitzer, Christoph Olscher, Xavier Monforte, Barbara Gepp, Elisabeth Simböck

Poster 29
Use of Assay Ready KeratinoSens® Cells to test for Skin Sensitization
Lukas Focke, Valerie de Boor, Andreas Natsch, Oliver Wehmeier

Poster 30
Refinement of an in vitro testing battery for developmental neurotoxicity assessment by integration of radial glia- and astrocyte-specific endpoints
Etta Zühr, Katharina Koch, Ellen Fritsche

Poster 31
Cross-species-relevance of in vitro bioassay battery for the assessment of thyroid hormone disruption
Jiri Novak, Puja Kumari, Runze Liu, Klara Hilscherova

Poster 32
Proteomics reveals mechanisms of metabolic disruptive effects of emerging di-(2-ethylhexyl) phthalate substitutes
Alexandra Schaffert, Isabel Karkossa, Elke Ueberham, Rita Schlichting, Katharina Walter, Josi Arnold, Matthias Blüher, John T. Heiker, Jörg Lehmann, Martin Wabitsch, Beate I. Escher, Martin von Bergen, Kristin Schubert

Poster 33
Cell type and developmental time-dependent effects of genotoxins on neural stem-/progenitor cell functions in vitro
Melanie Pahl, Jördis Klose, Ellen Fritsche

Poster 34
Retrofitting in vitro test systems for high throughput screening of thyroid disrupting chemicals with external biotransformation systems – a critical review and tiered in vitro/in silico approach
Sebastian Lungu-Mitea, Andreas Schiwy, Felipe Vidal, Inska Reichstein, Puja Kumari, Miriam N. Jacobs, Klara Hilscherova

Poster 35
Development of the NPC1ab_RAR_GR assay, an in vitro test method to identify putative endocrine disrupting chemicals (EDCs) perturbing glucocorticoid receptor (GR) or retinoic acid receptor (RAR) signaling
Kevin Schlüppmann (YSTA), Saskia Hüsken, Louisa Stark, Arif Dönmez, Eike Keßel, Nils Förster, Katharina Koch, Ellen Fritsche

Poster 36
Mitochondrial toxicity; AOP-driven extrapolation from in vitro/in silico data to regulation
Wanda van der Stel, Giada Carta, Julie Eakins, Johannes Delp, Ilinca Suciu, Anna Forsby, Andrea Cediel-Ulloa, Kristina Attoff, Florentina Troger, Hennicke Kamp, Iain Gardner, Barbara Zdrazil, Martijn J.Moné, Gerhard F. Ecker, Manuel Pastor, Jose Carlos Gómez-Tamayo, Andrew White, Erik H. J. Danen, Marcel Leist, Paul Walker, Paul Jennings, Susanne Hougaard Bennekou, Bob van de Water

Poster 37
A xeno-free in vitro 3D synovial membrane model mimicking the pathogenesis of arthritis
Julia Beißel, Christina Lubahn, Duc Ha Do Nguyen, Moritz Pfeiffenberger, Frank Buttgereit, Timo Gaber, Alexandra Damerau

Poster 38
Fluidic shear stress reduces TNFα-mediated cartilage damage in a 3D model of degenerative joint disease
Christina Lubahn, Julia Beißel, Duc Ha Do Nguyen, Moritz Pfeiffenberger, Thomas Leeuw, Frank Buttgereit, Timo Gaber, Alexandra Damerau

Poster 39
VHP4Safety: Assessing the safety of chemicals and pharmaceuticals without using laboratory animals
Anne S. Kienhuis, Cyrille A.M. Krul, Juliette Legler and the VHP4Safety consortium

Poster 40
Twinning towards excellence in alternative methods for toxicity assessment – TWINALT project
Zuzanna Sobańska, Joanna Roszak, Tamara Vanhaecke, Maria Dusinska, Elise Rundén-Pran, Marina Marinovich, Edyta Reszka

Poster Session 2

Wednesday, 28.9.2022, 14:00 – 15:30, first floor

Poster 41
A mouse pup model for learning the Phalanx Distalis Amputation and pup handling
Maximilian Nuber, Miriam Priester, Nadine Baumgart, Jan Baumgart

Poster 42
The role of the Animal Welfare Body in ensuring compliance with the 3Rs: a case study of the forced swim test
Kimberley Jayne, Julia Baines, Emily R Trunnell

Poster 43
Target group specific efforts to facilitate replacement of animal experimentation
Lisa Andersson, Kaisa Askevik, Elvira Hukasova, Emma Svensk

Poster 44
Transforming Culture: Culture of Care in Animal Research
Katharina Ameli,, Stephanie Krämer

Poster 45
Antibodies: Resistances to overcome on the way to a cruelty-free future
Johanna Walter, Tamara Zietek, Corina Gericke

Poster 46
Modernizing Medical Research to Benefit People and Animals
Isobel Hutchinson, Carla Owen, Jarrod Bailey

Poster 47
Knowledge, skills, attitudes and beyond
Nick D. Jukes

Poster 48
How to address animal methods bias in scientific publishing
Catharine E. Krebs, Helder Constantino, Lilas Courtot, Kathrin Herrmann, Ann Lam, Sofia Batista Leite, Janine McCarthy, Brinda Poojary, Kristie Sullivan

Poster 49
Attitudes towards animal dissection and animal-free alternatives among high school biology teachers in Switzerland
Miriam A. Zemanova, Silvia Frey

Poster 50
TPI.tv: A visual platform for animal-free innovations
Victoria C. de Leeuw, Harm J. Heusinkveld, Anne S. Kienhuis

Poster 51
ReThink3R 2.0 Summer School – Educating the next generation of scientists
Annemarie Lang, Lisa Grohmann,Katharina Hohlbaum, Philipp Burt, Alexandra Damerau, Marcel Bermudez, Moritz Pfeiffenberger, Laura Behm

Poster 52
Animal and Tissue Exchange platform (ATEX)
Jan van der Valk, Daan Weustenraad, Wim de Leeuw

Poster 53
Knowledge and Attitude Towards laboratory animals use and 3Rs: A cross-Sectional Study in Sudan
Ahmed Algali, Abednego N. Masai, Fadlalbaseer A. E. Alnoor, Saeed S. A. Ahmed, Ragda A. A. Mohammed, Ali A. Saeed, Banu C. Tel

Poster 54
Combining antiviral, anti-inflammatory and antibacterial properties of drugs in fighting COVID-19 disease
Jana Viskupičová, Petronela Rezbárikova, Marta Šoltesová Prnová, Helena Kandarova, Magdalena Májeková

Poster 55
Establishment of a human Synovium-on-a-chip for osteoarthritis disease-modeling using animal-free technologies
Eva I. Reihs (YSTA), Reinhard Windhager, Stefan Toegel, Hans P. Kiener, Peter Ertl, Mario Rothbauer

Poster 56
Single Cell Sequencing To Study IL-1β Induced Changes In Chondrocyte Pellet Cultures
Sebastian Kurmies (YSTA), Antje Vester, Marta Ferreira-Gomes, Gabriela M. Guerra, Frederik R. Heinrich, Carl C. Götzke, Vikram Sunkara, Pawel Durek, Joel D. Boerckel, Mir-Farzin Mashreghi, Annemarie Lang

Poster 57
A human in vitro model for airway epithelial injury and regeneration
Ashesh Chakraborty (EPAA student grantee), Michal Mastalerz, Hannah Marchi, Raphael Meixner, Rudolf A. Hatz, Jürgen Behr, Michael Lindner, Anne Hilgendorff, Claudia A. Staab-Weijnitz

Poster 58
Human-derived in vitro test systems of inflammatory bowel diseases as an alternative to laboratory animal models
Spyridon Damigos, Christina Fey, Nicolas Schlegel, Marco Metzger, Daniela Zdzieblo

Poster 59
Advanced in vitro models to study molecular mechanisms of Alzheimer’s disease
Zahra Motamed, Carine Gaiser, Laura Suter-Dick

Poster 60
Cultivation of Human Skin Biopsies for Preclinical Testing on human trabecular meshwork cells.
Astrid Wurbs, Katrin S. Wallner, Clemens Kittinger, Georg Singer, Beate Rinner

Poster 61
Enhanced drug metabolisms of hepatocytes with physiological aerobic respiration enabled by a new oxygen-permeable plate as an alternative culture format
Yasuyuki Sakai, Masaki Nishikawa, Hiroyasu Ito, Fumiya Tokito, Mathieu Danoyl, Takumi Kawanishi, Hiroshi Arakawa, Yukio Kato, Tomoaki Matsugi, Jingjing Yang, Katsuhiro Esashika, Toru Sumita

Poster 62
Vitamins’ B6 and C enhance the huIFN-αn3 induction by the NDV ZG1999HDS or Sendai virus
Bratko Filipič, Lidija Gradišnik, Adriana Pereyra, Hrvoje Mazija

Poster 63
Direct Replacement of Secondary Antibodies by Affimers
Isobel Gibson

Poster 64
Neutralising effects of different antibodies on Clostridioides difficile toxins TcdA and TcdB in a translational approach
Georg Csukovich (YSTA), Nina Kramer, Barbara Pratscher, Ivana Gotic, Patricia Freund, Rainer Hahn, Gottfried Himmler, Sabine Brandt, Iwan A Burgener

Poster 65
High throughput intestinal tissues and on newly fabricated 96-well culture plates
Zachary Stevens, Paul Kearny, Mitch Klausner, Alex Armento, Marek Puskar, Seyoum Ayehunie

Poster 66
Human placental villous tissue explants as basis for 14-day toxicity studies
André Schmidt, Enora Flache, Astrid Schmidt, Nora Schulz, Maren Klett, Merle-Johanna Küstner, Frank Weise, Jörg Hampl, Gregor Schlingloff, Andreas Schober, Udo R. Markert

Poster 67
Expression dynamics of pregnane X receptor-controlled genes in 3D primary human hepatocyte spheroids
Tomas Smutny, Veronika Bernhauerova, Lucie Smutna, Jurjen Duintjer Tebbens, Petr Pavek

Poster 68
Implementing microfluidic systems into the 2D and 3D cultures: challenges and benefits of the flow
Peter Pôbiš, Helena Kandarova

Poster 69
3D lung-organoid platform for the identification of pharmaceutical compounds targeting senescence and inflammation
Michelle Brand, Felix Ritzmann, Deivydas Milasius, Yiwen Yao, Rolf Müller, Robert Bals, Daniela Yildiz, Christoph Beisswenger

Poster 70
Differentiation of specialized epithelial cells in porcine intestinal organoids
Judith Lehmann, Maren Schenke, Bettina Seeger

Poster 71
Antibiotics free nanoformulations for treatment of non–healing wounds
David O. Oluwole, Lucy Coleman, William Buchanan, Tao Chen, James Adams, Roberto M. La Ragione, Lian X. Liu

Poster 72
Development, efficacy and safeyt of a novel therapeutic protocol against an eperimental models of human malignant melanoma
Anestopoulos Ioannis, Paraskevaidis Ioannis, Tragkola Venetia, Kyriakou Sotiris, Panayiotidis Mihalis

Poster 73
Anti-hyperglycemic, anti-hyperlipidemic, anti-inflammatory and antioxidant activity of Amachol®, an aqueous amaranth seed extract, using a human small intestinal organotypic culture model
Sonia Scarfì, Stefania Vernazza, Serena Mirata, Jan Markus, Silvia Letasiova, Alessia Mattiello, Roberto Premoli, Anna Maria Bassi, Mirko Magnone

Poster 74
Use of Assay Ready THP-1 derived macrophages to test for pyrogen contamination
Adrian Dittberner, Lukas Focke, Mirta Jacobs, Karen Hinsch

Poster 75
Evaluation of ocular side effects of systemically adminstered medications using the in vitro reconstructed human corneal epithelial tissue model
Yulia Kaluzhny, Mitchell Klausner, Viktor Karetsky, Alex Armento, Marek Puskar

Poster 76
International Project Train-SafeMDs: Towards the development of an in vitro testing strategy for local tolerance testing of medical devices used in the oral cavity
Helena Kandarova, Dagmar Jírová, Winfried Neuhaus, Kristína Kejlová, Markéta Dvořáková, Lada Svobodová, Alena Moulisová, Marian Rucki, Ana Špilak, Peter Pôbiš

Poster 77
Evaluation of phototoxic potential and ability to form ROS of selected compounds by using EpiDermTM Phototoxicity test
Martina Nalezinkova, Lenka Hudecova, Jan Markus, Silvia Letasiova

Poster 78
Ex vivo platform for feeding of ticks
Matěj Kučera, Tereza Hatalová, David Hartmann, Jan Perner

Poster 79
A Promising Method for the Determination of Cell Viability: The Membrane Potential Cell Viability Assay
Eneko Madorran, Andraž Stožer, Zoran Arsov, Uroš Maver, Jan Rožanc

Instructions for Posters

Please see Practical Information for Presenters & Speakers for more information.