The 2024 European Congress on Alternatives to Animal Testing

Replacement

• Disease models and microphysiological systems
• Emerging technologies: stem cells incl. hiPSCs, bioprinting, spheroids, organoids, multi-organ models, “-omics” analysis, systems biology approaches, IT and big data
• Biological barriers: e.g. lung, gut, kidney & skin epithelia, blood-brain & blood-saliva barriers
• Animal-free human based cell culture – replacement of serum, matrigel, gelatin, collagens, trypsin, S9, etc. – and recombinant antibodies
• Good cell culture practice
• Development and implementation of Novel Approach Methodologies (NAMs)
• In silico models: toxicology & efficacy of drugs, chemicals & cosmetics, new approaches for biomedical research
• In vitro toxicology: Specific endpoints of toxicity, alternatives to animal testing in food safety and nutrition, Neurotoxicity & Developmental Neurotoxicity (DNT) and Ecotoxicology
• Efficacy and safety testing of drugs, medical devices, biopharmaceutics, cosmetics, consumer products (e. g. toys) and chemicals (REACH & alternatives)

Reduction

• Preregistration, reporting, and experimental design
• Organ and tissue sharing and approaches to reduce surplus animals
• Translational aspects (species differences) and reproducibility

Refinement

• Best practice approaches and culture of care
• Avoidance of severe suffering and pain management/assessment
• Cage enrichment and non-invasive handling
• AI – Artificial Intelligence to advance the Three Rs and human centered research and testing: research/screening tools, creation and use of virtual animal and human populations for pre- and clinical studies, translation tool from animals to humans
• NGRA – Next generation risk assessment
• Animal research ethics: 3R, Harm-Benefit Analysis, ethical review
• 3Rs in education and academia
• 3Rs centres and platforms in Europe & international, national and local centres
• 3Rs and policy: Implementing 3Rs guidelines and concepts, Novel Approach Methodologies (NAMs) and Directives – Ethical and legal issues – Cosmetics testing ban (and REACH)
• 3Rs research funding
• COST Action IMPROVE: 3Rs concepts to improve the quality of biomedical science
• “Young Scientists” session
• Publication policies – ARRIVE guidelines & Co

Tuesday, 17th September 2024

3Rs centres WORKSHOP organized by COST Action IMPROVE

13:00 – 20:00: Workshop in the Loft of the MedCampus Linz

Detailed information and registration to be found via following link:

https://cost-improve.eu/event/3rs-centres-workshop

Wednesday, 18th September 2024

10:00: Start of Registration

11:30 – 12:30: YOU Event – Meet-up for Young Scientists (Hall 3)

13:00 – 13:15: Opening

13:15 – 13:45: Keynote lecture (Hall 1), Paul Carmichael (Unilever): “NAMs and NGRA: It’s all going to end in Tiers”, Chairs: Horst Spielmann & Winfried Neuhaus

14:00 – 15:30 and 15:45: Scientific sessions in Hall 1, Hall 2, Hall 3

Hall 1: Session “NAMs and NGRA implementation in industry”, sessions chairs: Dilyana Filipova & Abdulkarim Najjar

14:00 – 14:20: Abdulkarim Najjar: “Next Generation Risk Assessment: Mechanistic Insights and Implementation of NAMs for Toxicokinetics and Toxicodynamics Characterization”

14:20 – 14:40: Thomas Bertelsen: “Governance for ensuring Replacement at Novo Nordisk, a global pharmaceutical company”

14:40 – 15:00: Aurélie Thomas CANCELLED: “Reducing our reliance on animals through advanced technologies: today and tomorrow”

15:00 – 15:15: Laurence Walder: “Key elements for transitioning to non-animal science in the pharmaceutical sector”

15:15 – 15:30: Anne Kienhuis: “From research and development to uptake of New Approach Methodologies (NAMs): a landscape approach”

15:30 – 15:45: Dilyana Filipova: “Why do drugs keep failing? A systematic analysis of drug failure rates from 1963 to 2017”

Hall 2: Session “Inflammation & Infection models”, sessions chairs: Bettina Seeger & Christoph Wiesner

14:00 – 14:20: Bettina Seeger: “Venezuelan equine encephalitis virus (VEEV) infection during early brain development impairs neuronal migration in hiPSC-derived neurospheres”

14:20 – 14:40: Eva Reihs: “Engineering of a tissue-mimetic biochip model of the human synovial membrane for osteoarthritis research using non-animal-derived protocols and materials”

14:40 – 15:00: Puja Pandey: “Characterization of Alternative Methods for Intestinal Epithelial Cell Regeneration after Infection” – YSTA awardee

15:00 – 15:15: Christoph Wiesner: “An Optogenetic Approach to Investigating the Molecular Function of TLR4 and TLR10 in Mesenchymal Stem Cells”

15:15 – 15:30: Charlotte von Heckel: “Establishment of three-dimensional infection and cancer tissue models for the validation of laser-based diagnostic medical devices”

Hall 3: Session “Animal Methods Bias”, sessions chairs: Kathrin Herrmann & Melanie Ort

14:00 – 14:20: Catharine Krebs: “Animal methods bias and the COLAAB-orative effort to overcome it”

14:20 – 14:40: Ignacio Tripodi: “Inspecting the scientific literature for signs of animal methods bias”

14:40 – 15:00: Melanie Ort: “Animal methods bias in education”

15:00 – 15:15: Celean Camp: “Reviewer guidelines and trainings to mitigate animal methods bias”

15:15 – 15:30: Emily Trunnell: “Findings of a workshop to explore animal methods bias in funding”

15:30/15:45 – 16:15: Coffee break

16:15 – 18:00: Scientific sessions in Hall 1, Hall 2, Hall 3

Hall 1: Session “Methods for Toxicology”, sessions chairs: Klara Hilscherova & Christopher Faßbender

16:15 – 16:35: Klara Hilscherova: “In vitro assays for the assessment of thyroid hormone disruption – comparison of different models and detection methods”

16:35 – 16:55: Petra Labohá: “Towards the validation of an in vitro assay for gap junctional intercellular communication to identify non-genotoxic carcinogens”

16:55 – 17:15: Alexandra Schaffert: “A Comprehensive Approach to Cardiotoxicity Assessment using Adverse Outcome Pathways”

17:15 – 17:30: Christopher Faßbender: “Developing Integrated Approaches to Testing and Assessment for acute fish toxicity”

17:30 – 17:45: Christian Schlechtriem: “Development and implementation of the Hyalella azteca bioconcentration test (HYBIT)”

17:45 – 18:00: Christoph Schür: “Machine learning-based prediction of fish acute mortality”

Hall 2: Session “Animal-free human based cell culture”, sessions chairs: Tilo Weber & Jeffrey Bajramovic

16:15 – 16:35: Jeffrey Bajramovic: “An integral approach to replace animal-derived products for cell culture”

16:35 – 16:55: Tilo Weber: “A new animal-product-free, defined, and universal cell culture medium: Easy to use, do-it-yourself and beneficial for 2D and 3D culturing of normal and cancer cells”

16:55 – 17:15: Joachim Wiest: “Evaluation of chemically defined cell culture media with the cellasys #8 assay”

17:15 – 17:30: Sara Santos: “Advanced allogeneic porous scaffolds for personalized xeno-free cell culture and tissue engineering”

17:30 – 17:45: Beat Thalmann: “First ever animal-free, cell line-derived human S9 fraction for in vitro drug metabolisation”

17:45 – 18:00: Johannes Hackethal: “Translation and Reproducibility – Standardized HUMAN Biomaterials”

Hall 3: Session “3R Research Funding in Europe”, sessions chairs: Herwig Grimm & Martijn Nolte

16:15 – 16:35: Herwig Grimm: “The Swiss National Research Programme «Advancing 3R – Animals, research and society» (NRP 79)”

16:35 – 16:55: Martijn Nolte: “A Dutch perspective on funding the transition towards animal-free innovations”

16:55 – 17:15: Erica van Oort: “Non-animal approaches in biomedical research and testing of pharmaceuticals: an ERA Policy Agenda proposal”

17:15 – 17:30: Jenny Sandström: “Promoting Applied 3Rs Research – Insights from Switzerland”

17:30 – 17:45: Sandra Paschkowsky: “Funding 3R research in Germany – a key to new innovative methods”

17:45 – 18:00: Kristina Wagner: “What have we achieved? – retrospective assessment of 3Rs projects funded by the set foundation”

18:30 – 21:00: Welcome Reception (Aula)

20:00 – 21:00: YOU Event – Meet the Mentor (Hall 3 – Seminar room first floor)

Thursday, 19th September 2024

9:00 – 9:30: Keynote lecture (Hall 1), Pandora Pound: “Rat Trap: A popular science book about the limitations of animal research and the technologies available to replace it”, Chairs: Merel Ritskes-Hoitinga & Arti Ahluwalia

9:40 – 11:25: Scientific sessions in Hall 1, Hall 2, Hall 3

Hall 1: Session “The Value of Transition Approaches in Animal Free Scientific Research”, sessions chairs: Merel Ritskes-Hoitinga & Ingrid van Visseren

09:40 – 10:00: Love Hansell: “Safety Assessment through Animal Free Evolution (SAFE): The Role of Transformative Governance in the Transition to Animal-Free Regulatory Reseach”

10:00 – 10:20: Anne Kienhuis: “Pathways to regulatory acceptance of New Approach Methodologies for Endocrine-Disrupting Chemicals”

10:20 – 10:40: Phil Macnaghten: “Valuing Testing: Understanding societal and stakeholder perspectives in developing and validating animal-free models for toxicological studies”

10:40 – 11:00: Giorgia Pallocca: “Toward Regulatory Transformation in the Biological and Chemical Sectors Globally: Progress and Challenges”

11:00 – 11:25: Panel discussion

Hall 2: Session “Stem cell models”, sessions chairs: Verena Charwat & Horst Spielmann

09:40 – 10:00: Nina Graffmann: “Induced pluripotent stem cell (iPSC)-derived hepatocyte like cells: An essential tool for disease modelling and toxicity tests”

10:00 – 10:20: Maximilian Schinke: “Generation of Human Sensory Macrophages to Replace Animal Research in Preclinical Pharmacology and Drug Safety Application”

10:20 – 10:40: Verena Charwat: “Arrhythmogenic Drug Evaluation in a High Throughput 3D Cardiac Microtissue System”

10:40 – 10:55: Nicole Schupp: “hiPSC differentiating into renal proximal tubular cells are more sensitive to nephrotoxins and oxidants than iPSC and iPSC differentiated into renal proximal tubular cells”

10:55 – 11:10: Gianluca Ciardelli: “3D cardiac tissue models for pollutants toxicity testing”

11:10 – 11:25: Petra Stahlschmidt-Allner: “Induced Pluripotent Stem Cells vs Healthy Fish Stem Cells for Comprehensive in vitro Toxicology”

Hall 3: Session “Culture of Care: Enhancing Communication among Stakeholders for 3Rs Progress “, sessions chairs: Fernando Gonzalez Uarquin & Katharina Ameli

09:40 – 10:00: Katharina Ameli: “Transforming Culture: Culture of Care in Animal Research focussing communication”

10:00 – 10:20: Thomas Bertelsen: “Culture of Care at Novo Nordisk, a pharmaceutical company – caring for animals and people”

10:20 – 10:40: Paul Friedemann Pohlig: “Fostering Effective Communication and Collaboration in Animal Research Facilities”

10:40 – 10:55: Adrian Smith: “Culture of Care: where are the resources I need to improve conditions at my workplace?”

10:55 – 11:10: Barney Reed: “How good communication can advance a good culture of care”

11:10 – 11:25: Fernando Gonzalez Uarquin: “Appreciation is key: improving the 3Rs by optimizing communication with young researchers”

11:25 – 11:45: Coffee break

11:45 – 13:15: Scientific sessions in Hall 1, Hall 2, Hall 3

Hall 1: Session “Cancer models”, sessions chairs: Beate Rinner & Jens Kurreck

11:45 – 12:05: Beate Rinner: “Modeling glioblastoma: from primary tumors to relapse and the use of post-mortem sample for translational research”

12:05 – 12:25: Jens Kurreck: “Bioprinting of vascularized cancer model for drug testing”

12:25 – 12:45: Michael Ausserlechner: “3D bioprinted tumor-on-chip models to study tumor microenvironment for drug testing and disease modelling”

12:45 – 13:00: Karolina Pokrzywka: “Do extracellular vesicles from mesenchymal stem cells inhibit cancer development?”

13:00 – 13:15: Pedro Pinto: “Novel Microfluidic Prostate-Cancer Model: a tool to a study MicroRNA secretion and their potential as diagnostic biomarkers”

Hall 2: Session “Novel in silico tools”, sessions chairs: Sharon Bryant & Arti Ahluwalia

11:45 – 12:05: Sharon Bryant: “The NeuroDeRisk 3R In Silico Toolbox: De-Risking Neurotoxicity and 3D-Pharmacophores”

12:05 – 12:25: Luca Gerardo-Giorda: “Digital twinning as an alternative to animal experimentation in the treatment planning of cardiac arrhythmias”

12:25 – 12:45: Chiara Magliaro: “Digitoids: a novel computational platform to assess the metabolic-electrophysiological behaviour of neurons cultured in monolayers”

12:45 – 13:00: Beata Ondrusova: “Framework for identifying the origin of premature ventricular contractions using patient-specific electrocardiographic imaging”

13:00 – 13:15: Ermes Botte: “Between Heisenberg and Schrodinger: a novel approach to quantify measurement-induced perturbations in cell cultures”

Hall 3: Session “3Rs Implementation: Progress and Barriers”, sessions chairs: Barney Reed & Masaki Nishikawa

11:45 – 12:05: Shujun Cheng: “Regulatory Advances in China Alternative Toxicology Methods”

12:05 – 12:25: Masaki Nishikawa: “JSAAE Activities toward the formation of the Asian Federation”

12:25 – 12:45: Barney Reed: “Supporting Replacement in Academia: Examining the social and cultural barriers and opportunities around the acceptance and uptake of non-animal methods”

12:45 – 13:00: Kathrin Herrmann: “Germany’s political goal of “reducing animal experimentation” – are we making progress?”

13:00 – 13:15: Julia Pochat: “From the meaning of words to the power of actions: What changed for animals in laboratories in the last EU political term and what can we expect from the new term?”

13:15 – 14:00: Lunchtime – Business Lunch sponsored by

14:00 – 15:30: Poster session 1 (first floor)

15:30 – 17:15: Scientific sessions in Hall 1, Hall 2, Hall 3

Hall 1: Session ” 3Rs: ethical conceptual considerations and harmonisation”, sessions chairs: Monique Janssens & Johan Lauwereyns

15:30 – 15:50: Dominik Hajosi: “Mission impossible accomplished? On the incoherent implementation of the harm-benefit analysis as a requirement of Directive 2010/63/EU”

15:50 – 16:10: Monique Janssens: “What would Miffy do? Applying informed consent by proxy to all sentient animals”

16:10 – 16:30: Nico Müller: “Monkeys and moonshots: Exploring mission-oriented policies for animal-free research”

16:30 – 16:45: Johan Lauwereyns: “Toward an expansive interpretation of the 3Rs across areas of animal use”

16:45 – 17:00: Leah Haut: “Dealing with surplus animals – What to do instead of killing millions of animals annually in Europe”

Hall 2: Session “Outer Biological Barriers”, sessions chairs: Helena Kandarova & Shujun Cheng

15:30 – 15:50: Hanna Glasebach: “Novel 3D in-vitro psoriasis model based on specific genetically engineered keratinocytes” – YSTA awardee

15:50 – 16:10: Claus-Michael Lehr: “Human in vitro models for developing drugs against bacterial infections of lung and skin”

16:10 – 16:30: Sabina Eibichova: “Application of silicone flow bioreactors for dynamic cultivation of in vitro and ex vivo corneal models”

16:30 – 16:45: Helena Kanďárová: “Assessment of cytotoxicity, skin and oral irritation of medical devices with hygroscopic properties. Case study”

16:45 – 17:00: Mahmoud Galal: “CAMSkin Model: Re-vascularized Human Skin after Cultivation on the Chorioallantoic Membrane”

Hall 3: Session “Refinement”, sessions chairs: Özge Selin Cevik & Györgyi Szabo

15:30 – 15:50: Annemarie Lang: “Repeated injection of sustained-release buprenorphine for pain management in mouse femoral fracture models”

15:50 – 16:10: Katharina Hohlbaum: “Lockbox enrichment—manipulative and cognitive activities for laboratory mice”

16:10 – 16:30: Thomas Bertelsen: “Non-aversive handling of research animals at Novo Nordisk”

16:30 – 16:45: Karolina Krehl: ”Rumination behavior as an animal-based indicator in sheep”

16:45 – 17:00: Özge Selin Cevik: “Enhancing anxiety behavior research in rodents with network meta-analysis”

17:00 – 17:30: Coffee break

17:30 – 19:00: Round table/panel discussion (Hall 1, ground floor)

with 17:30 – 17:45: Johan Lauwereyns: “Can we agree on what the 3Rs mean?”

20:00 – 24.00: Gala dinner

Friday, 20th September 2024

9:00 – 9:30: Keynote lecture (Hall 1), Anna-Dorothea Gorki (GST Antivirals): “Of mice and men: new tools to study respiratory tract infections”, Chairs: Winfried Neuhaus & Claus-Michael Lehr

9:40 – 11:10: Scientific sessions in Hall 1, Hall 2, Hall 3

Hall 1: Session “Lung models”, sessions chairs: Emily Reis & Claus-Michael Lehr

09:40 – 10:00: Stefan Hippenstiel: “Microenvironmental acidification by pneumococcal sugar consumption fosters barrier disruption and immune suppression in the human alveolus”

10:00 – 10:20: Mariana Guedes: “Establishing a lentiviral reporter platform for screening 3D human lung organoids: a proof-of-concept approach” – EPAA and YSTA awardee

10:20 – 10:40: Tobias Neu: “The novel monoclonal human alveolar epithelial cell line “Arlo” for modeling the pulmonary air-blood-barrier in vitro” – YSTA awardee

10:40 – 10:55: Ludovica Cacopardo: “In silico-in vitro design of biomimetic alveolar models for high-throughput inhalation tests”

10:55 – 11:10: Emily Reis: “Development and characterization of reconstructed tracheo-bronchial tissue models from multiple species”

Hall 2: Session “Policy / networking”, sessions chairs: Anne Kienhuis & Winfried Neuhaus

09:40 – 10:00: Thomas Bertelsen: “The power of Animal Welfare Body networks to promote best practices”

10:00 – 10:20: Anne Kienhuis: “Interdisciplinary and multisectoral collaboration to stimulate acceptance of the Virual Human Platform for Safety Assessment (VHP4Safety)”

10:20 – 10:40: Jennifer Rosowski: “Emerging networks in Berlin: Charité 3R, Der Simulierte Mensch and Einstein Center 3R”

10:40 – 10:55: Alexandra Schaffert: “Innovative Animal-Free Approaches in Regulatory Toxicology: The OECD Advisory Group on Emerging Science in Chemicals Assessment – ESCA”

10:55 – 11:10: Winfried Neuhaus: “The COST-ACTION Networking Activity IMPROVE 3Rs Concepts to Improve the Quality of Biomedical Science”

Hall 3: Session “3Rs Implementation: Providing frameworks and tools”, sessions chairs: Kristina Wagner & Athanassia Sotiropoulos

09:40 – 10:00: Yiguang Zhu: “Advancing Validation Methodologies and Regulatory Acceptance of Microphysiological Systems”

10:00 – 10:20: Kristina Wagner: “The German Animal Welfare Federation’s “Guide to Animal-free science” – a comprehensive overview of scientific and ethical progress”

10:20 – 10:40: Bettina Bert: “SMARFIRA – Finding the needle in a haystack”

10:40 – 10:55: Athanassia Sotiropoulos: “French Centre for 3Rs: Promotion of Open Science”

10:55 – 11:10: Mareike Ahrends: “Introducing the HTCR Foundation: A Non-Profit Biobank of Human Biospecimens for 3R-Aligned Biomedical Research”

11:10 – 11:40: Coffee break

11:40 – 13:10: Scientific sessions in Hall 1, Hall 2, Hall 3

Hall 1: Session “Emerging technologies I”, sessions chairs: Philipp Paulitschke & Markéta Dvořáková

11:40 – 12:00: Maddalena Collini: “A platform for in vivo label-free tissue histology to reduce the use of animals in biomaterial tests”

12:00 – 12:20: Philipp Paulitschke: “Advancing Cell-Based Assay Reproducibility Through Lensfree Imaging and AI Integration”

12:20 – 12:40: Juan Bejarano Rios: “Development of a bioreactor for dynamic culture and laser based non-destructive measurements of tissue models” – YSTA awardee

12:40 – 12:55: Francesco Fontana: “Multiscale and multimodal characterization of thymus structure and mechanics for biomimetic design approaches” – YSTA awardee

12:55 – 13:10: Isidora Loncarevic: “Optimizing endpoints for crystalline quartz silica exposure in a lung co-culture model” – YSTA awardee

Hall 2: Session “Musculoskeletal In vitro models”, sessions chairs: Annemarie Lang & Frank Schulze

11:40 – 12:00: Jonathan Strauss: “Advancement of human osteoarthritic cartilage-on-a-chip models by integration of glucose and lactate biosensors for monitoring of disease-related shifts in cell metabolism” – YSTA awardee

12:00 – 12:20: Anne Bernhardt: “3D printed in vitro bone models”

12:20 – 12:40: Katharina Wirsig: “Quadruple culture of primary human osteoblasts, osteocytes, osteoclasts and endothelial cells – an advanced 3D in vitro bone model” – YSTA awardee

12:40 – 12:55: Annemarie Lang: “Implementation of an in vitro angiogenesis assay to study mechanisms of vascular invasion during endochondral ossification”

12:55 – 13:10: Frank Schulze: “Development of a microfluidic perfusion bioreactor that enables culture of multiple 3D bone models under mechanical loading”

Hall 3: Session “Reduction: Planning and Reporting”, sessions chairs: Julia Menon & Adrian Smith

11:40 – 12:00: Sibylle Wenzel: “Even beyond animal testing – possibilities for an overall asessment of animal well-being”

12:00 – 12:20: Adrian Smith: “Quality, fast, cheap: choose two. Practical advice on how to conduct better science”

12:20 – 12:40: Tilo Weber: “Have the non-technical summaries of animal experiments in Europe improved? An update”

12:40 – 12:55: Julia Menon: “Preregistration of animal studies improves reporting quality”

12:55 – 13:10: Julia Menon: “Better Science through More Transparency – a mixed-methods case study of the Dutch’s research landscape”

13:10 – 14:00: Lunchtime

14:00 – 15:30: Poster session 2 (first floor)

15:30 – 17:00: Scientific sessions in Hall 1, Hall 2, Hall 3

Hall 1: Session “Emerging technologies II”, sessions chairs: Valeria Chiono & Mario Rothbauer

15:30 – 15:50: Mohammad Ghosheh: “A Microphysiological System for Simultaneous Neural and Oxygen Measurement in Brain Tissues Using Integrated Opto-Electrical Sensor System”

15:50 – 16:10: Valeria Chiono: “In vitro models of human cardiac fibrosis on biomimetic nanofibrous scaffolds: a novel preclinical testing platform for precision nanomedicine”

16:10 – 16:30: Gerardina Ruocco: “Engineered fiber-based anisotropic microenvironments for 2D and 3D in vitro models of skeletal muscle tissues” – YSTA awardee

16:30 – 16:45: Barbara Wolff-Winiski: “Patient-specific assays to identify and characterize new drugs for chronic wound therapy”

16:45 – 17:00: Mario Rothbauer/Eva Reihs for discussion afterwards: “Biomechanically loaded three-dimensional tissue-mimetic lab-on-a-chip models for human inflammation and fibrosis modelling”

Hall 2: Session “Inner organ models”, sessions chairs: Christina Fey & Nathalie Jung

15:30 – 15:50: Masaki Nishikawa: “Gas-permeable membrane-based direct oxygenation enables cellular aerobic respiration both in static culture and in perfusion microphysiological systems”

15:50 – 16:10: Ahmed Ali: “3D Bioprinting of Humanized Xeno-free Liver Models” – YSTA awardee

16:10 – 16:30: Christina Fey: “Synthetic fibrous scaffold-based 3D small intestinal mucosa in vitro model”

16:30 – 16:45: Nathalie Jung: “Exploring nanoparticle-tissue interactions in in vitro models of the intestinal mucosa in health and disease”

16:45 – 17:00: Adrián Klepe: “Challenges and insights of investigating small extracellular vesicle internalisation and permeation across the blood-brain barrier in vitro” – YSTA awardee

17:00 – 17:15: Pilar Samperio Ventayol: “A model to study human intestinal epithelium response to probiotics”

Hall 3: Session “Education about 3Rs”, sessions chairs: Nick Jukes & Debby Weijers

15:30 – 15:50: Monique Janssens: “Developing a Global Education Hub for animal-free methods”

15:50 – 16:10: Nick Jukes: “Case studies of innovation and replacement to illustrate the trajectory of an ideal, fully humane veterinary degree”

16:10 – 16:30: Maren Hülsemann CANCELLED: “Contribution of human organ models to COVID-19 research – A large-scale systematic review”

16:30 – 16:45: Maude Everaert: “RE-Place: a tool to stimulate the use of New Approach Methodologies in Belgium”

16:45 – 17:00: Debby Weijers: “Empowering researchers to take the leap from lab to business to increase the impact of animal-free innovations”

17:00 – 17:15: Maoling Wei: “A brief introduction to 3Rs education in China”

17:30 – 18:00: YSTA Awards/Announcement WC13/Farewell

POSTER numbers for THURSDAY, 19.9.2024 and FRIDAY, 20.9.2024 can be found via following link:

EUSAAT – Poster Sessions EUSAAT 2024 congress

Poster Session 1

Thursday, 19.9.2024, 14:00 – 15:30, first floor

Poster 1 | Abstract Number 220
Norecopa: PREPARE for better Science
Adrian Smith

Poster 2 | Abstract Number 29
Establishing a culture of care environment through knowledge exchange – a project to increase mutual understanding leading to better results in health and research
Patrizia Gibler

Poster 3 | Abstract Number 243
Moral Case Deliberation (MCD) sessions within an animal facility
Sophie Schober

Poster 4 | Abstract Number 53
The Effect of Clicker Training on the Acute Physiological Stress Response in C57BL/6J Mice
Dorothea Pichl

Poster 5 | Abstract Number 104
Addressing pitfalls in animal behavior measurement through refinement in anxiety studies with meta-analysis
Özge Çevik

Poster 6 | Abstract Number 75
Recommendations for scientific fish husbandry
Bianka Grunow

Poster 7 | Abstract Number 156
Indicators for 3R monitoring in Switzerland
Aude Rapet

Poster 8 | Abstract Number 128
Innovation in Action: Collaboration between the Austrian 3R Center and Government Initiatives Birgit
Reininger-Gutmann

Poster 9 | Abstract Number 92
3R-Competence Network North Rhine-Westphalia – The alliance for medical progress in line with the best possible animal welfare
Michael To Vinh

Poster 10 | Abstract Number 210
3Rs in focus – how to make researchers more aware?
Györgyi Szabó

Poster 11 | Abstract Number 173
The introduction of alternative in vitro methods in the laboratory practicals
Tijana Markovič

Poster 12 | Abstract Number 97
A manifesto from the young generation advocating to go animal testing-free
Julia Menon

Poster 13 | Abstract Number 195
New paving stones for the road towards an animal free regulatory system: INSIGHT and CHIASMA
Martin Paparella

Poster 14 | Abstract Number 90
Prioritizing non-animal methods to improve environmental risk assessment for pharmaceuticals and promote innovation in the EU
Christopher Faßbender

Poster 15 | Abstract Number 196
Combining Education and Research: Replacement of Fetal Calf Serum for routine cell culture purposes
Jeffrey Bajramovic

Poster 16 | Abstract Number 147
Start the transition to animal-free antibodies for your research
Esther Wenzel

Poster 17 | Abstract Number 50
MONOCYTE ACTIVATION TEST (MAT) AS POTENTIAL ASSAY FOR QUALITY CONTROL OF VETERINARY AUTOGENOUS VACCINES: PRELIMINARY DATA
Rosario Liberti

Poster 18 | Abstract Number 8
Enhancing Atlantic Sturgeon Stem Cell Culture: Assessing Serum Reduction and Growth Factor Substitution Effects
Valeria Di Leonardo

Poster 19 | Abstract Number 167
Development of a defined foetal calf serum-free medium for differentiated HepaRG® cell culture (cancelled)
Agnes Jamin

Poster 20 | Abstract Number 69
Comparative Proteomic Characterization of serum-free cultivated HepG2 cells reveals upregulated drug metabolism and increased oxidative stress protection
Luisa-Marie Pfeifer

Poster 21 | Abstract Number 168
Designing new fluorescent probes for efficient monitoring of drug-induced liver injury effects on bile canaliculi motility and albumin expression
Pamela Bachour-El Azzi

Poster 22 | Abstract Number 123
Effect of nephro- and hepatotoxins on precision cut tissue slices as a potential 3R-compliant method to assess organ toxicity
Nicole Schupp

Poster 23 | Abstract Number 241
Adverse outcome pathways to unravel drug-induced toxicity
Charline Sommer – YSTA awardee

Poster 24 | Abstract Number 82
Establishment of 3D FCS-free cultures of human proximal tubule epithelial cells
Stan Zuidervaart – YSTA awardee

Poster 25 | Abstract Number 184
Proliferation and differentiation capacity of an in vitro cell line model for myelopoiesis adopted to chemically-defined media
Pascal Woitschewski

Poster 26 | Abstract Number 77
Advancing Ethical and Reproducible Stem Cell Culture: Alternatives to Chemically Undefined Animal-Derived Matrices in in vitro Toxicology Testing
Julia Spänle

Poster 27 | Abstract Number 126
Animal free biomimetic fibrous scaffolds for the setup of 3D multi-layered epithelial models
Christina Fey

Poster 28 | Abstract Number 56
Non-invasive Monitoring of Three-Dimensional Tumor Spheroids using Electrical Impedance Spectroscopy
Ahmed Khattab

Poster 29 | Abstract Number 232
Unveiling the Dynamics of Scratch Assays: Continuous, Label-Free Monitoring of Cell Migration Using Lensfree Imaging and AI
Anna Jötten

Poster 30 | Abstract Number 187
Evolvoid: A Biophysical-Inspired Genetic Algorithm for Optimal Cellular Construct Design towards Lab-on-a-Laptop
Flavio Fontana

Poster 31 | Abstract Number 198
Nebuloid: a novel in silico agent-based cell model
Piera Mancini

Poster 32 | Abstract Number 201
Latest advancements in the use of in silico methods for the qualification of non-genotoxic impurities
Christian Novello

Poster 33 | Abstract Number 137
Animal-free optimization of energy delivery in cardiac Radiofrequency ablation: A virtual human study
Minha Anees

Poster 34 | Abstract Number 72
Modeling and analysis of standard-of-care vs. experimental, oncogene-specific therapeutic protocols in human digestive system cancers organoids
Albert Gandurski

Poster 35 | Abstract Number 141
MUG-hOSEC: A Human Ex Vivo Skin Model Breaking Boundaries
Beate Rinner

Poster 36 | Abstract Number 136
Autologous co-culture tumor models: Novel Therapeutic Approaches for Cholangiocarcinoma
Christina Karner

Poster 37 | Abstract Number 131
Autologous co-culture tumor models: „Novel Therapeutic Approaches for Clear Cell Sarcoma
Djenana Vejzovic – YSTA awardee

Poster 38 | Abstract Number 160
Autologous co-culture tumor models: Correlation of lipid signatures with drug resistance in CIC::DUX4 sarcoma cell line
Djenana Vejzovic – YSTA awardee

Poster 39 | Abstract Number 245
Evaluation and Integration of Blood-Brain Barrier In Vitro Models in a Safety and Sustainability Assessment Framework within the project CHIASMA
Duygu Turan Sorhun

Poster 40 | Abstract Number 79
Exploring Neuron-Mediated Chronic Pain: Insights from In vitro Modelling for Pharmacology and Toxicology
Kathrin Lämmerhirt

Poster 41 | Abstract Number 148
Stem cell-derived human brain tissue to study invasion mechanisms of primary brain tumors
Youcef Dahmani

Poster 42 | Abstract Number 154
Optimization of the synaptosome model to reduce tissue requirement and its application to examine the role of neural RAS
Zoltán Lincmajer

Poster 43 | Abstract Number 207
Water soluble Propolis Greit 120 and HuIFN-αN3 shows anti – Influenza virus activity in vitro
Bratko Filipič

Poster 44 | Abstract Number 46
Innovative therapy of influenza and respiratory infections caused by viruses through the use of siRNAs (shortinterfering RNAs)
Aurora De Mattia

Poster 45 | Abstract Number 25
Towards porcine enteroid cultures for rotavirus studies
Elena Ferrari

Poster 46 | Abstract Number 247
Development of an alternative method to animal experiments using a physical stimulation model for osteoarthritis
Katsuko Furukawa

Poster 47 | Abstract Number 44
Investigating the systemic distribution of implant wear products in ovo using the hen`s egg as a model
Laura Selle

Poster 48 | Abstract Number 226
Development of an in vitro test method for irritation of medical devices used in the oral cavity
Marek Puskar

Poster 49 | Abstract Number 158
Development of experimental setup based on a tissue-engineered 3D-cell phantom to evaluate Electroporation
Noah Müller

Poster 50 | Abstract Number 48
An in vitro method for anti-hair loss efficacy testing
Shujun Cheng

Poster 51 | Abstract Number 49
An in vitro method for anti-glycation efficacy in cosmetics
Shujun Cheng

Poster 52 | Abstract Number 162
In vitro phototoxicity assays – limitations and benefits of a combined approach to compound testing
Ulrike Theisen

Poster 53 | Abstract Number 165
Impedance-based identification of severe and mild eye irritation in vitro
Nicola Knetzger

Poster Session 2

Friday, 20.9.2024, 14:00 – 15:30, first floor

Poster 1 | Abstract Number 76
Human stem cell-derived neurospheres as a model to characterize the impact of Listeria monocytogenes infection on early brain development
Annika Fischer – YSTA awardee

Poster 2 | Abstract Number 155
The investigation of synaptic monoamine uptake in rat brain synaptosomes
Dóra Lóska

Poster 3 | Abstract Number 186
DNT-RAP4: Pioneering Non Animal Methods-Based DNT Risk Assessment of Pesticides engaging EFSAs AOP based IATA approach via four new case studies
Ishita Virmani

Poster 4 | Abstract Number 117
Myelin matters: Development of a human stem cell-based peripheral myelin toxicity assay for regulatory testing
Lisa Haiber

Poster 5 | Abstract Number 78
Body-on-a-Chip Technologies Towards Neurotoxic Monitoring and Environmental Safety/CBRN Applications
Mateo Vasconez Martinez

Poster 6 | Abstract Number 179
Measuring metabolism in whole-brain organoids
Sonia Cerchio

Poster 7 | Abstract Number 130
Integration of the Blood-Brain Barrier and Brain Organoids in a Modular Microfluidic Platform for Sensor-Based Analysis in Drug Screening and Disease Modeling Applications
Sophie Schweinitzer – Physicians Committee for Responsible Medicine – YSTA awardee

Poster 8 | Abstract Number 224
Translation of an Established in vitro Blood-Brain Barrier Differentiation Protocol to CRISPR/Cas9 Gen-Edited, Patient-derived hiPSC Lines: Highlighting Challenges Nikoletta
Kardos-Török – YSTA awardee

Poster 9 | Abstract Number 249
Label-free functional analysis for the characterization of iPSC-derived neural organoid development and maturation
Timm Schlegel

Poster 10 | Abstract Number 250
Evaluation of plate-to-plate reliability in a label-free cytotoxicity assay for dose response analysis
Timm Schlegel

Poster 11 | Abstract Number 40
In-vitro ion-channel dependent neurotoxicity evaluation using a series of Ion-channel stable overexpression cells
Shujun Cheng

Poster 12 | Abstract Number 57
IN-DEPTH PROTEOMIC PROFILES OF HIPSC-DERIVED EARLY RETINAL ORGANOIDS
Anna Bassi

Poster 13 | Abstract Number 98
Development of a dual-flow microfluidic device to study the interaction of the intestinal epithelium and the enteric nervous system
Alexandra Lorenz

Poster 14 | Abstract Number 251
P38 MAPK as a potential target to prevent IL-6/IL-22 induced leaky gut in Caco-2 cell model
Nikolai Leinz

Poster 15 | Abstract Number 182
Mucoadhesive magnetic nanoparticles for restoration of intestinal function in childhood visceral myopathy
Paolo Signorello

Poster 16 | Abstract Number 225
In vitro 3D reconstructed models of human duodenum, jejunum and ileum
Silvia Letasiova

Poster 17 | Abstract Number 222
EpiOcularTM time-to-toxicity – a test method for subcategorization of eye irritants
Silvia Letasiova

Poster 18 | Abstract Number 223
In vitro reconstructed model of human epidermis containing melanocytes for studying skin pigmentation and lightening
Silvia Letasiova

Poster 19 | Abstract Number 150
Transcriptomic Profiling of Native versus Human Skin Equivalent (cancelled)
Eva-Anne Subileau

Poster 20 | Abstract Number 93
Measurement and simulation of transdermal delivery of topical drug formulations in a dynamic microfluidic diffusion chamber in health and disease
Franciska Erdő

Poster 21 | Abstract Number 70
Differences between potential skin and respiratory sensitizers tested by non-animal methods
Eliska Pacalova

Poster 22 | Abstract Number 176
Production of Fibroblast Coated Sutures and Examination of Their Applicability – Preliminary Study
Levente Goschler

Poster 23 | Abstract Number 228
Toxicological aspects of in vitro oral mucosa wound healing models
Marek Puskar

Poster 24 | Abstract Number 233
Eye damage reversibility in an in vitro model of bovine cornea to replace the Draize Test completely
Martina Benedetti

Poster 25 | Abstract Number 240
NOVEL PROTOCOL FOR IN VITRO BIOCOMPATIBILITY EVALUATION OF INTRAORAL MEDICAL DEVICES
Peter Pôbiš

Poster 26 | Abstract Number 229
Performance evaluation: incorporating GARD®skin assay into the OECD TG 497 Defined Approaches as a KE3 component
Tim Lindberg

Poster 27 | Abstract Number 1
JNK is activated in an Egfr-induced Drosophila lung tumor model and promotes cancer progression
Leizhi Shi

Poster 28 | Abstract Number 161
Optimization of a 3D multi-layer cell culture to study NSCLC chemoresistance mechanisms
Giulia Paties Montagner

Poster 29 | Abstract Number 140
Use of public transcriptional datasets for validating pulmonary in vitro models
Lorenz Latta

Poster 30 | Abstract Number 6
Quantitative AOP modeling of mucus hypersecretion using 3D cells for comparative risk estimation of tobacco products
Sakuya Ichikawa

Poster 31 | Abstract Number 36
From Flat to 3D Bioprinted Cell Cultures: Revolutionizing Breast Cancer Studies
Dorottya Moldvai

Poster 32 | Abstract Number 31
In vitro patient-derived tumour models – innovative 3D bioprinted long-term tissue cultures to test drug sensitivity
Dániel Sztankovics – YSTA awardee

Poster 33 | Abstract Number 9
Keeping an Eye on the Move: Tracking Cell Migration with Optical Imaging in 3D
Irem Duman – YSTA awardee

Poster 34 | Abstract Number 52
Targeting mitochondrial defects in a 3D-bioprinted LCHADD/VLCADD model
Antonia Degen

Poster 35 | Abstract Number 4
Systematic analysis of gut bacterial carcinogen biotransformation and its functional consequences
Boyao Zhang

Poster 36 | Abstract Number 105
Development of a modular fluid system to investigate the permeation of drugs from different BCS classes through an epithelial barrier to predict pharmacokinetics
Verena Vogel

Poster 37 | Abstract Number 144
Fluid dynamics optimisation in flow-through permeation test systems
Dorottya Kocsis

Poster 38 | Abstract Number 30
3D human Vascular-on-a-Chip: Method development for the biological assessment of endothelial dysfunction and monocytes migration
Ayaka Hayashida

Poster 39 | Abstract Number 51
Coculture of Jurkat and THP-1 cell lines as a model for the evaluation of immunosuppressive properties of chemicals
Nina Franko

Poster 40 | Abstract Number 127
A replacement approach to animal model for the toxicity evaluation of autogenous vaccines
Martina Checco

Poster 41 | Abstract Number 163
Revealing Molecular Mechanisms of SARS-CoV-2 Spike Protein in Renal Epithelium through Microphysiological Modeling (MPS)
Maryna Somova

Poster 42 | Abstract Number 208
Assessing Male Reprotoxicity of Real-Life Mixtures Using Novel Approach Methodologies
Eliška Sychrová

Poster 43 | Abstract Number 209
Novel pull-down assay for efficient separation and identification of endocrine disruptors in complex environmental samples
Zuzana Toušová

Poster 44 | Abstract Number 175
Utilization of human lymphoblasted cell lines as an in vitro test system to unveil immunotoxicity of bisphenol A analogues and their mixtures
Tijana Markovič

Poster 45 | Abstract Number 74
Utilising experimental NAMs to assess genomic instability in liver models
Michael Burgum

Poster 46 | Abstract Number 65
HepG2 spheroids: A new testing approach for the determination of adverse effects of chemicals
Tim Ravnjak

Poster 47 | Abstract Number 63
Investigation of complex in vitro models using shotgun proteomics for the prediction of human-relevant data for anti-inflammatory drugs
Simone Lichtner

Poster 48 | Abstract Number 134
3DTransfect – multi-scale drug-target validation by transfected 3D cell cultures
Vytaute Starkuviene

Poster 49 | Abstract Number 206
Multilayered nano-hydroxyapatite (nHA)/collagen (Coll) coated-PLGA scaffolds as in vitro tools to model metastatic prostate cancer in the bone and evaluate drug performance
Katie Ryan

Poster 50 | Abstract Number 45
Investigating pathological mechanical strain in ligament disease
Johannes Heidenberger – YSTA awardee

Poster 51 | Abstract Number 255
In vitro skeletal muscle model responsive to free fatty acids: A promising tool for testing toxins and metabolites
Xiaoying Chen

Poster 52 | Abstract Number 189
Daphnids as Early Warning Systems for Pharmaceutical Contamination: A Metabolomic Approach
Emma Rowan – EPAA awardee

Poster 53 | Abstract Number 200
The “Fish invitrome” as an animal-free alternative for the assessment of organ-specific toxicity in fish – a focus on environmental neurotoxicity
Jessica Bertoli

Poster 54 | Abstract Number 214
A composite bioink derived from lacrimal gland extracellular matrix and alginate
Luis Grumm

Instructions for Posters

Please see Practical Information for Presenters & Speakers for more information.